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In the past, the streptavidin-biotin reaction has been used to immobilize biological molecules on a solid support, such as an immobiovalent solid phase. For example, the streptavidin-biotin reaction has been used to immobilize antibodies, hormones, and enzymes on solid supports. See, for example, U.S. Pat. No. 5,437,987 (Ohkawa et al.) and U.S. Pat. No. 4,678,827 (Ejaz).
Another method for immobilizing biological molecules on a solid support is the ligand-exchange method. The ligand-exchange method involves the covalent linkage of a biological molecule to a reactive group on a solid support, which subsequently reacts with a reactive group on another biological molecule to form a high molecular weight complex. See, for example, U.S. Pat. No. 4,182,646 (Ruther et al.).
Yet another method for immobilizing biological molecules on a solid support is the self-immobilization method. The self-immobilization method involves immobilization of the biological molecule by reacting it with a biological molecule possessing a reactive group in the absence of a solid support. See, for example, U.S. Pat. No. 5,482,867 (Markwell et al.).
Another method for immobilizing biological molecules on a solid support is the cross-linking method. The cross-linking method uses a chemical reagent, such as glutaraldehyde, to crosslink proteins. See, for example, U.S. Pat. No. 4,421,770 (
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