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Category:Video game franchises introduced in 2010DESCRIPTION (from applicant’s abstract): The long-term goal of this research is to determine which genetic mechanisms contribute to the cellular autonomy of cells expressing c-myc (i.e., transformed cells). The post-doctoral training proposed will allow the applicant to integrate approaches from genetics, biochemistry, cell biology, and genetics to study the biology of c-myc expression and its possible involvement in gene regulation in mammalian cells. The specific aims of the project are to: (1) determine the effect of trans-acting and cis-acting DNA binding factors on c-myc transcription; (2) define cis-acting DNA sequences within the mouse mammary tumor virus (MMTV) promoter that interact with transcription factors to allow myc expression to occur at high levels; (3) identify trans-acting factors that can alter expression of the c-myc gene in transient transfection assays; and (4) identify trans-acting factors that alter c-myc expression in stably transformed cell lines. A widely used approach to study the activity of genes within mammalian cells is to express trans-acting factors that can alter the activity of the genes of interest in culture. This approach has been extremely successful with the c-myc gene. However, the majority of these studies have relied on over-expression of trans-acting factors (by either viral or chemical induction). With this approach, the activity of the gene is severely perturbed and is difficult to interpret. The proposed research will use a new system to study the activity of genes by expressing factors in stable cell lines. This approach makes it possible to study the activity of genes during steady-state expression. Such studies will allow the investigator to learn how the activity of the genes is controlled within a cellular environment that mimics the environment in which the genes function in vivo. In addition, certain factors that alter the expression of the genes of interest in transient expression assays will be assessed for their ability to alter the expression of the genes in vivo.School of Arts and Sciences
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