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A water-in-oil-in-water adjuvanted poliovirus vaccine was developed as an inactivated polio vaccine (IPV). It was produced using 0.8 M ammonium sulfate, at which point a small number of viable virions were found to have survived the suspension process. Viable virus was recovered by formaldehyde treatment to a final titer of 7.0 log10 PFU/mL and the recovery of the empty capsid protein was confirmed by Western blot analysis. This virus was compared to a concurrently produced vaccine with an inert adjuvant to analyze the mechanism(s) for inactivation. A one-step removal of ammonium sulfate with subsequent growth in the presence of a low dose of formaldehyde at 37 degrees C inactivated the inert vaccine and did not inactivate the IPV. This suggests that the inactivation of the IPV is due to the presence of ammonium sulfate, that the virus is sensitive to ammonium sulfate, and that the inactivation mechanism is adsorption-associated. The characterization of the inactivated vaccines included testing for potency, sterility, pyrogenicity, and the absence of whole virus, viral RNA and protein, and genomic RNA integrity using PCR, dot blot hybridization, and reverse transcriptase-PCR (RT-PCR), respectively. Sufficient vaccine to inoculate 20-30 monkeys was prepared for each vaccine lot. The vaccine is to be incorporated into the United Nations’ Expanded Program on Immunization schedule of World Health Organization’s Regional Office for Africa, and the manufacture of the additional doses of IPV will be undertaken by state-licensed manufacture-controlling laboratories.Barnes & Noble

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