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Anoctamin-1 (ANO1) is a transmembrane ion channel that is expressed throughout the body, especially in the skin, brain, and renal tissues. Although ANO1 has been shown to possess conductance properties and voltage-dependent regulation, a physiologic function has not yet been demonstrated. TRPV3 has recently been identified as an osmosensitive channel which is ubiquitously expressed throughout the body. We have tested the hypothesis that ANO1 functions as an inhibitor of TRPV3 channel function. To test this hypothesis, we co-expressed ANO1 and TRPV3 in HEK-293 cells and monitored calcium (Ca2+) flux in response to hypoosmotic swelling. We observed dose-dependent inhibition of the swelling response by co-expression of ANO1 with TRPV3. The hypoosmotic swelling response in cells expressing TRPV3 alone was similar to that observed in HEK-293 cells expressing the channel alone. Additionally, co-expression of ANO1 with TRPV3 had little effect on TRPV3 regulation by 2-aminoethoxydiphenylborate and guanine nucleotide. We conclude that
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