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Splicing is thought to be the most likely source of the dramatic divergence in coding and non-coding regions of eukaryotic genomes. In Saccharomyces cerevisiae, the intron-containing pre-mRNA splicing intermediates differ from the corresponding mature mRNA in their size and their ability to be polyadenylated. Therefore, one of the major goals of the field is to elucidate the biochemical reactions that control the efficiency and fidelity of pre-mRNA splicing. A model for the maturation of the intron containing pre-mRNA, encoding the poly(A) binding protein (PABP) involved in RNA trafficking and translation, has been developed based on the well-characterized in vivo kinetics of its splicing. By combining this model with in vitro measurements of transcription-associated events, we have identified the rate limiting step that regulates alternative splicing, polyadenylation and exon ligation. This study has also revealed an unexpected and yet highly efficient mechanism to remove poly(A) tails from intron containing pre-mRNAs. The RNase III orthologous to mammalian Dicer, Rnt1p, interacts with the complex that binds PA sites, itself comprising the Ccr4/Pop2/Not complex, in a fashion reminiscent of the interaction of Dicer with other complexes. We propose that Rnt1p unwinds the pre-mRNA to release the RNA from the splicing complex for degradation by the exosome.AR Review: Benchmark’s Artificial Intelligence Dungeon Crawler
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The penetration of cefotaxime into rat bone and bone marrow was studied after intravenous injection. Cefotaxime was found in all tissues examined with the highest concentrations of drug being recovered from bone. The greater penetration of cefotaxime into bone than into soft tissue may be accounted for by the fact that bone is a metabolically active tissue and by the slow rate of diffusion of the drug through bone. Tissue concentrations of cefotaxime at a dose of 100 mg/kg were found to be significantly higher than those achieved with ceftazidime at a dose of 30 mg/kg, in the same range of time, with the possible explanation that cefotaxime is a more suitable antibiotic for the treatment of osteomyelitis. that the spectral properties of two-dimensional photonic crystals can be constructed from the [*eigenstates*]{} of three-dimensional photonic crystals, i.e. [*bound states*
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