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Cell surface macromolecular structures of human spermatozoa are regularly described in relation to their fertilizing capacity. Such observations have led to the suggestion that the well-known fertilizing ability of human spermatozoa may be related to the presence of certain antigens at the surface of these cells. Our own investigations have failed to confirm this relation. However, we have found that the antibodies used in previous experiments may have reduced the fertilizing capacity of spermatozoa. Therefore, in order to reveal a possible relationship between the presence of sperm cell surface structures and the fertilizing capacity of human spermatozoa we compared two groups of individuals with widely differing fertilizing capacity. The sperm cells in these groups were free of antibodies as were the antibodies used in the present investigation. One group consisted of men whose semen did not fertilize within 60 h, while the other group consisted of men whose semen fertilized the oocytes within 30 min. Both groups were divided into A and B subgroups. In A subgroups, antibodies to sperm antigens were added to the sperm suspension before insemination; in the B subgroups they were added to the oocyte suspension before insemination. The effect of this procedure was studied using computer assisted sperm analysis. The non-fertilizing semen samples from group A revealed oligospermia. The high percentage of immobilized spermatozoa from group A indicates that these semen samples may contain several layers of spermatozoa with different adhesiveness properties. In the B subgroups the percentage of immobilized spermatozoa was significantly reduced. These results are in agreement with our previous observations, indicating the important role of the macromolecular structures of spermatozoa in penetrating the cumulus oophorus. The results also indicate that the analysis of these structures by means of sperm surface-specific antibodies may be a useful adjunct in the evaluation of the fertilizing ability of human spermatozoa.Full Suite Joomla Component
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