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Directed by Manmohan Singh. With Jimmy Sheirgill, Neeru Bajwa, Guggu Gill, Om Puri. A romantic comedy set in the lush fields of Punjab and the beautiful cityÂ .
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Cytosine methylation in plant genomes contributes to the regulation of gene expression. Although several genes are transcribed at the site where they are chemically methylated, studies of 5-methylcytosine (5mC) in mature plant tissues have been limited to large numbers of uniformly expressed (housekeeping) genes. To investigate whether 5mC levels vary among genes, we used methylated DNA immunoprecipitation (MeDIP), a technique for enrichment of methylated genomic DNA, to compare 5mC levels between up-regulated and down-regulated genes of Medicago truncatula. A comparison of chromatin 5mC content of differentially expressed genes by MeDIP indicated that 17% of the hypomethylated genes were up-regulated (11 of the 59 genes
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