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Immunocytochemistry on cultured neurons {#s4-3}
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Cultures of ventricular rat neurons were prepared as described in ([@bib43]). Briefly, animals (postnatal day 0–2, females) were decapitated under isofluorane anesthesia, brains were removed and sectioned in the ventricular-subventricular zone. Neurons were dissociated and incubated in a high glucose (25 mM) DMEM (Invitrogen) supplemented with N2 (1%), 2-mercaptoethanol (50 μM), penicillin (50 μg/ml), streptomycin (50 μg/ml) and B27 (1:50) at 37°
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